Interleukin-18 and miR-130a in severe sepsis patients with thrombocytopenia
نویسندگان
چکیده
BACKGROUND Thrombocytopenia is one of the most common laboratory abnormalities encountered in patients with severe sepsis. It has been reported that thrombocytopenia is linked to mortality in patients with severe sepsis. However, the mechanism of thrombocytopenia in sepsis is unknown. We hypothesized that inflammatory cytokines and microRNAs (miRNAs) are not only involved in the pathogenesis of sepsis, but also are correlated with thrombocytopenia. PATIENTS AND METHODS Eligible patients with severe sepsis were prospectively recruited and treated at our hospital between June 2012 and May 2014. The miRNA and protein expression of interleukin (IL)-18 and IL-27 were detected by real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. The expression of miR-130a and miR-150 was detected by TaqMan real-time polymerase chain reaction. RESULTS Sixty eligible patients were divided into two groups: 28 severe sepsis patients with thrombocytopenia and 32 severe sepsis patients without thrombocytopenia. The results demonstrated that the miRNA expression and plasma concentration of IL-18 in severe sepsis patients with thrombocytopenia were higher than those in severe sepsis patients without thrombocytopenia (P=0.015 and P=0.034, respectively), and miR-130a expression was significantly lower in severe sepsis patients with thrombocytopenia (P<0.003). CONCLUSION Our data demonstrate that severe sepsis patients with thrombocytopenia have increased plasma and miRNA expression levels of IL-18 and decreased expression of miR-130a, suggesting that IL-18 and miR-130a might be involved in the pathophysiological process of severe sepsis with thrombocytopenia.
منابع مشابه
Petechial Hemorrhage: A clinical diagnosis of neonatal Thrombocytopenia and sepsis
A preterm female baby with birth weight of 1.5kg was referred to our hospital on day 6 for difficulty in breathing. Baby was admitted at birth for respiratory distress and feed intolerance to other hospital and in view of clinical deterioration baby was referred. Baby had thrombocytopenia with platelets counts of 11000/ mm3 and high CRP titer. Baby had petechial haemorrhagic spots all over the ...
متن کاملThrombocytopenia and Associated Factors in Neonates Admitted to NICU during Years 2010_2011
Abstract Background Thrombocytopenia is the most common hematological abnormality which is encountered in the neonatal intensive care unit (NICU). The incidence in neonates varies greatly, depending upon the population studies. According to the frequency of thrombocytopenia and its complications and because of lack of such research in Iran, this study was performed on neonates admitted to Sha...
متن کاملmiR-142-5p and miR-130a-3p are regulated by IL-4 and IL-13 and control profibrogenic macrophage program
Macrophages play a pivotal role in tissue fibrogenesis, which underlies the pathogenesis of many end-stage chronic inflammatory diseases. MicroRNAs are key regulators of immune cell functions, but their roles in macrophage's fibrogenesis have not been characterized. Here we show that IL-4 and IL-13 induce miR-142-5p and downregulate miR-130a-3p in macrophages; these changes sustain the profibro...
متن کاملThe Prevalence and Risk Factors for Neonatal Thrombocytopenia among Newborns Admitted to Intensive Care Unit of Aliasghar Children’s Hospital
Background: Determining the prevalence and risk factors of thrombocytopenia among neonates enables us to prevent the inevitable and sometimes irreversible complications. In this study we aimed to survey the frequency of thrombocytopenia and its demographic features and risk factors. Materials and methods: In this cross sectional study the platelet counts of 364 neonates admitted to neonatal ...
متن کاملNeonatal thrombocytopenia in the NICU of our series: etiology, predisposing factors, management and short-term outcome
Background: Thrombocytopenia (TTP) is one of the most common neonatal hematological disorders. The causes of neonatal TTP are very various, leading to large differences in the clinical profile of the affected neonates. Therefore, the aim of this study was to investigate the characteristics, clinical diagnoses and short-term outcomes of neonatal TTP in Bahrami Children's Hospital. Material and ...
متن کامل